Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: CyTOF antibody panel

Article Snippet: Vio Bright FITC anti-human CXCR3 , Miltenyi Biotec , Cat# 130-118-673; RRID:AB_2734057.

Techniques:

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: Selected genes involved in Tfh cell biology

Article Snippet: Vio Bright FITC anti-human CXCR3 , Miltenyi Biotec , Cat# 130-118-673; RRID:AB_2734057.

Techniques: Activation Assay

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: Distinct CD4 + T cell subsets contribute to the generation of Tfh with heterogeneous functional profiles (A) Mean fluorescence intensity of the CXCR5 marker expressed by CXCR5 + PD-1 + cells derived from (1) naive, (2) MemPD-1 neg , (3) MemPD-1 neg and Tfh. (B) Frequency of IL-21- and/or IFNγ-positive cells among CXCR5 + PD-1 + cells at day 3. (C – E) Representative flow plots showing CXCR3, ICOS, and CD40L expression by CXCR5 + PD-1 + cells (left panel) and frequency of CXCR3-, ICOS-, and CD40L-positive cells among CXCR5 + PD-1 + cells at day 3 (right panel). (F) Ex vivo cells or their respective Tfh D3 counterparts obtained after 3 days of splenocyte culture were co-cultured with autologous B cells for 7 days. (G) Box plots represent the frequency of CD27 + CD38 + cells among CD19 + cells, the concentration of total immunoglobulins and the absolute number of live B cells after co-culture. (H) Quantification of IgG1, IgG4, and IgA in the co-culture supernatants. Each symbol (A–H) represents an individual donor. (A–H) A Wilcoxon matched pairs test was performed, ∗, p < 0.05; ∗∗, p < 0.005; ∗∗∗, p < 0.001.

Article Snippet: Vio Bright FITC anti-human CXCR3 , Miltenyi Biotec , Cat# 130-118-673; RRID:AB_2734057.

Techniques: Functional Assay, Fluorescence, Marker, Derivative Assay, Expressing, Ex Vivo, Cell Culture, Concentration Assay, Co-Culture Assay

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet:

Article Snippet: Vio Bright FITC anti-human CXCR3 , Miltenyi Biotec , Cat# 130-118-673; RRID:AB_2734057.

Techniques: Cell Analysis, Purification, Virus, Recombinant, Blocking Assay, Antibody Labeling, Transfection, Software

Journal: Acta Pharmaceutica Sinica. B

Article Title: Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1 high CD11b + cells elimination in hepatocellular carcinoma

doi: 10.1016/j.apsb.2022.08.006

Figure Lengend Snippet: Anti-PD-L1 antibody enhanced infiltration of CD8 + T cells by increasing proportion of cDC1 cells that secrete CXCL9. (A, B) Flow cytometry plots of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + gated on CD3 + CD8 + cells in all 6 groups. (C–E) Relative quantification of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + cells gated on CD3 + CD8 + T cells. (F) Immunofluorescence staining of tumor tissue sections. CD8 + T cells were stained with Alexa Fluor 594, CXCL9 was stained with Alexa Fluor 488. Scale bar = 200 μm. (G) The percentages of CD8 + T cells and CXCL9 + spots in every field of view found in all different 6 groups; three separate fields from each mice and three mice from each group were calculated. ∗ P < 0.05, ∗∗ P < 0.01,∗∗∗ P < 0.001, one-way ANOVA with Tukey's post hoc tests. All data are means ± SEM. (H,I) Representative flow cytometry analysis of the frequency of CXCL9 gated from different cell types in tumor microenvironment. (J, K) Analysis of flow cytometric quantification of CD11c + CD11b − cDC1 cells gated on tumor infiltrating CD45 + cells. (L, M) H22 cells were inoculated subcutaneously into BALB/c mice. 12 days later, cryoablation or microwave-ablation was proceeded, with anti-mouse CXCL9 neutralizing antibody (10 mg/kg) or anti-mouse PD-L1 antibody (10 mg/kg) being infused via tail injection every 3 days. Anti-mouse IgG was used as negative control. Tumor volume and frequency of infiltrated CD8 + T cells were revealed. n = 6 for each groups, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, no significant difference, t- tests. All data are means ± SEM.

Article Snippet: Mouse CXCL9 was stained by Rabbit anti-Mouse FITC-CXCL9 Antibody (CUSABIO, P18340, Wuhan, China).

Techniques: Flow Cytometry, Quantitative Proteomics, Immunofluorescence, Staining, Injection, Negative Control

Journal: Cell Reports Medicine

Article Title: A vaccine targeting antigen-presenting cells through CD40 induces protective immunity against Nipah disease

doi: 10.1016/j.xcrm.2024.101467

Figure Lengend Snippet:

Article Snippet: After washing with FACS buffer, cells were incubated for 30 min at 4°C with the Live/Dead Fixable Aqua Stain (Thermo Fisher Scientific, cat# L34975), the antibody phenotyping panel (mCD45-BV605 (BD clone 30-F11), mCD3-AF700 (Sony Biotechnology, clone 17A2), mB220-BV711 (BD, clone RA3-6B2), CD138-PE/Dazzle594 (Sony Biotechnology, clone 281-2), mIgG-FITC (Sony Biotechnology, Poly4060), mIgD-APC-H7 (Sony Biotechnology, clone 11-26c.2a), mFAS-BV421 (BD, clone Jo2) and mGL-7-PC-7 (Biolegend, clone GL7)), and two anti-biotin mAbs (APC, Miltenyi Biotec (France) REA746, and PE, Sony Biotechnology clone 1D4-C5).

Techniques: Virus, Recombinant, Transfection, Enzyme-linked Immunospot, Labeling, Transgenic Assay, Software, Sequencing, Fast Protein Liquid Chromatography, Flow Cytometry